Certified Wound Ostomy Nurse (CWON) Practice Test

When is it appropriate to obtain an anaerobic culture?

1. For shallow wounds without necrosis

2. For wounds with a lot of eschar and crepitus

3. For any surface-level wound

4. Only when indicated by the physician

The correct answer is: For wounds with a lot of eschar and crepitus

Obtaining an anaerobic culture is most appropriate for wounds that exhibit significant signs of anaerobic infection, which often includes the presence of eschar and crepitus. These characteristics suggest an underlying environment conducive to anaerobic bacteria, such as Clostridium species, which thrive in low-oxygen conditions. When necrotic tissue is present and the wound demonstrates crepitus (a crackling sensation felt under the skin, often due to gas produced by bacteria), this indicates a serious infection that may involve anaerobes. This type of culture is specifically beneficial in these situations, as it allows for the detection of bacteria that might not be identified through standard aerobic cultures. In contrast, shallow wounds without necrosis do not typically harbor anaerobic bacteria and would not necessitate anaerobic cultures. Similarly, surface-level wounds generally do not exhibit the conditions required for anaerobic growth, making such cultures unnecessary. While the physician’s indication for culture is important, the presence of specific wound characteristics is a primary determinant for obtaining an anaerobic culture.